Q. How accurate is HIV 4th generation duo antigen/antibody test?

Answered by
Dr. Basti Bharatesh Devendra
and medically reviewed by iCliniq medical review team.
This is a premium question & answer published on Feb 21, 2022

Hello doctor,

I had unprotected sex with a CSW last month. Two days later, I got her tested at a reputed pathology laboratory for HIV 4th generation duo antigen/antibody test, HBsAg, and HCV. All tests were negative. I never had any of the HIV symptoms.

As it could have been a high-risk exposure, I got tested on the below dates, and all came back negative:

1. HIV 4th generation duo antigen/antibody Test - non-reactive (0.172) at 20th day after exposure.

2. HIV 4th generation duo antigen/antibody Test - non-reactive (0.172) at 31st day after exposure.

I have shared my reports as an attachment. How accurate are these results? Do I need any further testing?

Please guide.




Welcome to

I have checked your reports (attachment removed to protect the patient's identity). It is good that HIV 4th generation duo antigen/antibody test is negative after 31 days of exposure. The chances of HIV (human immunodeficiency viruses) tests coming positive later are almost nil. However, it would be better to go for an HIV antibodies test after three months of exposure to get relieved of anxiety.

Hello doctor,

Thank you for your suggestion.

I would like to know about the window period of the HIV 4th generation duo antigen/antibody test. Will a negative result at 45 days be considered conclusive?



Welcome back to

HIV 4th generation duo antigen/antibody test is considered almost conclusive after one month of exposure. But it is better to go for an HIV antibodies test after three months of exposure to be relieved of anxiety and definitive results.

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