I have undergone a wide range of HIV tests, all of which were negative except the Western blot test, which was indeterminate. What does that conclude?

Q. What does indeterminate result in Western blot mean?

Answered by
Dr. Rakesh Kumar Bahunuthula
and medically reviewed by iCliniq medical review team.
This is a premium question & answer published on Aug 12, 2022

Hello doctor,

I have undergone a wide range of HIV testing. The test values are - On 78, 88, 96, 110, 120, 126, 158, 164, and 168th day, the fourth generation antigen and antibody tests were all negative, and on the 32nd and 64th days, HIV(Human Immunodeficiency Virus) rapid test was negative. HIV 1 DNA (Deoxyribonucleicacid) PCR qualitative on 78, 96, and 110th days not detected. HIV1 RNA PCR qualitative at 110th day not detected. HIV 1 RNA (Ribonucleic acid) PCR (Polymerase Chain Reaction) quantitative viral load on 126th day and 168th day - target not detected. But the result of the Western blot test was indeterminate with the p24 week band seen, and the rest band was negative on the 120th day. I took the viral load, and it was negative too. Please help me know why the result was intermediate. Can indeterminate convert into positive later? Can all bands convert into positive later? I am very nervous. My marriage is planned. Should I go to the wedding or stop the marriage?



Welcome to icliniq.com.

I have read your query and understand your concern. As the tests beyond 12 weeks are negative, you can consider yourself not to have acquired the infection. Indeterminate result in Western blot is a false positive as it does not correspond with the other test results. The chance of indeterminate becoming positive is nil unless there is any further exposure. I hope this helps.

Thank you.

Hello doctor,

Thank you for the response.That was the only exposure I had in my life. After the indeterminate Western blot result, I took the viral load test, which was also negative. I have read it online that p24 is the first antibody the body produces after infection. Is it true? I am nervous sometimes. I may be late seroconverting, and my antibodies are producing slowly. Doctor, please let me know if the indeterminate Western blot test result has lately converted into positive reports.



Welcome back to icliniq.com.

I can understand your concern. Internet has a vast amount of information which lacks authenticity. It is better if you stick to the standard websites where the information is reliable. I can understand your anxiety and it is very common in people to have a fear of HIV (human immunodeficiency virus). I have not seen any cases turn from negative to positive after 12 weeks if there was no further exposure. I hope this helps.

Thank you.

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