iCliniq logo

Ask a Doctor Online Now

HomeAnswersHIV/AIDS specialistfourth generation hiv testIs it not necessary to do other tests if fourth generation test negative at 8th week?

Is HIV RNA PCR test conclusive at 12 weeks?

The following is an actual conversation between an iCliniq user and a doctor that has been reviewed and published as a Premium Q&A.

Medically reviewed by

Dr. Vinodhini J.

Published At March 11, 2020
Reviewed AtJuly 31, 2023

Patient's Query

Hi doctor,

I went for an HIV test at six weeks after low-risk exposure and get false positive. At eight weeks both fourth generation and HIV(Human Immunodeficiency Virus) RNA(Ribonucleic acid) PCR(Polymerase Chain Reaction) are negative. At 9 and 10 weeks rapid test is negative. At 12 weeks HIV RNA PCR is negative. Is it conclusive? My doctor told me no need for further tests.

Hello, Welcome back to icliniq.com.

To answer your query, I want to give reference of published research that, Although the reported sensitivity and specificity of fourth generation tests are more than 99% in numerous studies, there are several instances of discordance between screening and confirmatory techniques (Liu P et al. AIDS Research and Therapy 2016 13:1). False positivity can be due to user error or cross contamination, if there is ongoing some other illness eg. malaria, or if there is a history of blood transfusion, autoimmune diseases. A positive test result always needs to be routinely confirmed using a different type of test or testing kit. Fourth generation tests are more than 95% accurate at six weeks and this needs to be confirmed after three months with a second test. If the test in your case has been performed at three months after the exposure and is giving negative results, and you have not had another risk, you do not have HIV. Your fourth generation test and RNA PCR at 9 and 10 weeks and further 12 weeks negative RNA PCR, suffices to rule out infection. If you still have apprehension, get a repeat HIV antibody test at six months of exposure, no need to repeat it in between. Take precautions in the future (consistent and correct condom usage). I hope this helps.

Same symptoms don't mean you have the same problem. Consult a doctor now!

Dr. Ravinder K. Sachdeva
Dr. Ravinder K. Sachdeva

HIV/AIDS specialist

Community Banner Mobile
By subscribing, I agree to iCliniq's Terms & Privacy Policy.

Ask your health query to a doctor online

HIV/AIDS specialist

*guaranteed answer within 4 hours

Disclaimer: No content published on this website is intended to be a substitute for professional medical diagnosis, advice or treatment by a trained physician. Seek advice from your physician or other qualified healthcare providers with questions you may have regarding your symptoms and medical condition for a complete medical diagnosis. Do not delay or disregard seeking professional medical advice because of something you have read on this website. Read our Editorial Process to know how we create content for health articles and queries.

This website uses cookies to ensure you get the best experience on our website. iCliniq privacy policy