Fourth Generation Hiv Test

HIV is a sexually transmitted disease which is caused by human immunodeficiency virus. There are various tests to detect the presence of HIV antigens/antibodies. These tests have evolved over time. A fourth-generation test identifies HIV antibodies as well as p24 antigens from the sample. Therefore, this test is more sensitive and helps identify the infection much earlier than a third generation test.

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After how many days of exposure should I take an HIV test?

Query: Hi doctor, I have attached my HIV reports taken on the 90th and 188th day after exposure for your reference. Both the reports are negative. There was no further exposure. Should I be concerned?   Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to I have gone through your reports (attachments removed to protect the patient's identity). Your test results will be considered conclusively negative if there is no further exposure. I hope my answer helps in relieving your stress. Please do not hesitate to ask if there ...  Read Full »

Is it necessary to take fourth-generation test even if other HIV tests are negative?

Query: Hello doctor,I had protected exposure followed by P24 antigen after 33 days, fourth-generation P24 Ag, HIV 1 and 2 antibodies (CMIA) after 38 days, and then tridot after 93 days. All reports were negative for all the tests. As all the reports are negative and protected exposure, can my HIV status be...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to The answers to your queries are: As you mentioned that it was protected sex, and there was no break or slippage of the condom. The risk of acquisition of HIV in this scenario is non-existent. It is great that your HIV test report at both time points is negative. If the...  Read Full »

Are fourth generation HIV test results reliable?

Query: Hello doctor, I had unprotected intercourse once with CSW last year. I tested for rapid ICT and PA methods after six weeks of exposure. HIV (Ag/Ab) CMIA test after eight weeks, both negative. I started getting chronic diarrhea for the past six months. A viral Marker test was done (16 months later)...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to Your test results are negative for HIV. If there had been no further exposure, these would be considered conclusive. However, if there is any doubt about the same or test result, there is no harm in getting a repeat HIV antibody test. This will allay your anxiety. Sy...  Read Full »

Why do I get itching and burning sensation in hands with muscle pain after risky sexual exposure?

Query: Hello doctor, Before three months, I had unprotected sex with a sex worker in her monthly (menstrual) period. After sex, I noticed bleeding from my cut penis. After one week, pain subsided. And after a month, I had sex, and again noticed bleeding from penis wound. I again had protected sex with se...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to The recommendations are to get the fourth generation HIV rapid test at the third or fourth week of the exposure. If the result is negative, get a confirmatory HIV rapid antibody test at 90 days (three months) of last exposure. Your negative HIV test result at three mont...  Read Full »

Is it not necessary to do other tests if fourth generation test negative at 8th week?

Query: Hi doctor, I went for an HIV test at six weeks after low-risk exposure and get false positive. At eight weeks both fourth generation and HIV RNA PCR are negative. At 9 and 10 weeks rapid test is negative. At 12 weeks HIV RNA PCR is negative. Is it conclusive? My doctor told me no need for further t...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hello, Welcome back to To answer your query, I want to give reference of published research that, Although the reported sensitivity and specificity of fourth generation tests are more than 99% in numerous studies, there are several instances of discordance between screening and confir...  Read Full »

Screened for HIV after eight weeks of exposure. Is it reliable?

Query: Hi doctor, I did an HIV rapid screening after eight weeks of exposure, and the results came negative. I do not have any other symptoms of HIV. Is this result reliable? Do I need to do other tests?  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to The tests available for HIV diagnosis are highly sensitive and specific and gives accurate results. Majority of the people who are infected with HIV develop HIV antibodies by two to eight weeks. Approximately, up to 5 % develop seroconversion by 12 weeks. Your HIV negat...  Read Full »

Can I get HIV through the cuts in the finger or sores in the mouth?

Query: Hi doctor, I am a 27-year-old male. I met a girl recently. We both did deep kissing and fingering many times. I do not know about her HIV status. Recently, I read about HIV, and I am worried. I do not remember whether I had any cut in my finger. Can I have HIV through the cuts in the finger or sore...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to Fingering alone will not cause HIV transmission. For transmission, the virus needs to enter the bloodstream. Regarding the cut, there should be a fresh lesion for having an active transfer. As far as deep kissing is concerned, there is no possibility to have HIV transmi...  Read Full »

My HIV tests at five weeks are negative. What are the chances for it to be positive later?

Query: Hello doctor, I had a 13 day HIV DNA PCR test, 28 day antibody test and a fifth week HIV fourth generation duo test. All the tests came back negative. What are my chances of still encountering an HIV positive test?  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hello, Welcome to Unprotected vaginal intercourse carries a risk of 4 in 10,000 exposures. Approximately 95 % of the people who are infected with HIV, develop HIV antibodies by four to six weeks of the exposure and almost all who are infected with HIV develop HIV antibodies by three ...  Read Full »

Do I have a risk of getting HIV from a drug addicted sex worker?

Query: Hi doctor, I took HIV RNA TMA TEST after six days and seven hours of exposure, and it was negative. But I am still worried. Can this result be a false negative? What about the accuracy of this test?  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to Although the test is sensitive, you have taken this test too early from the day of last exposure. You need to get retested after two weeks of exposure with the same test. I want to tell you the HIV test recommendations: The recommendations are fourth-generation HIV rap...  Read Full »

My CD4/CD8 ratio is 1.06. Does it indicate a HIV infection?

Query: Hello doctor, I had a possible high-risk exposure four months back, and I am worried about HIV infection. I got a fourth generation PCR, PCR for HIV2, and viral load, which were all normal. I still have symptoms like diarrhea and joint pain. Just last week I did a CD4 panel, and this is the resul...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hello, Welcome to Let me answer your questions step by step. A negative fourth generation test and HIV RNA PCR at four months is highly conclusive that you are HIV (human immunodeficiency virus) negative. Diarrhea and joint pains could have multiple reasons, not necessarily HIV. In fact...  Read Full »

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