Hiv Rna Pcr Test

HIV RNA PCR test is a type of test to detect HIV much earlier than the conventional tests. This test is also used to measure the activity of the HIV virus in a HIV positive person.

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All the answers published in this website are written by Verified medical doctors, therapists and health experts. The Content has been moderated by iCliniq medical review team before publication. Post your medical clarifications on iCliniq by choosing the right specialty and get them answered. Your medical queries will be answered 24/7 by top doctors from iCliniq.

The fourth generation combo test is negative. Am I risk-free from HIV?

Query: Hello doctor, I had protected vaginal sex and unprotected oral sex with CSW and for peace of mind, I tested with HIV fourth generation duo combo (Ag/Ab) test and RT PCR viral load test at 23 days from exposure. The fourth generation combo test came negative and RT-PCR viral load came below the detec...  Read Full »


Dr. Sushil Kakkar

Answer: Hi, Welcome to icliniq.com. Protected sex is safe as far as risk of HIV is concerned. Insertive or sex has a negligible risk of transmission of HIV. As far as testing is concerned, only a four-week fourth generation HIV screening test is considered reliable. Though even that has to be followed up ...  Read Full »

Which HIV test can be done nine days after possible exposure?

Query: Hello doctor, I did deep kissing like nine days ago with a girl that had HIV. Of course, I knew that after. And while it was dark, I was kissing her body too but because I did not have condom we did not have sex. Is it possible to get HIV by kissing her body or contacts with a body rash or something...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have noted your concern. Kissing is safe as far as the risk of HIV is concerned unless she had oral sores or bleeding gums which may increase the risk. Kissing on the skin is also not a risk. HIV is only transmitted through unprotected sex - either vaginal, anal ...  Read Full »

While on PEP, is HIV RNA PCR test conclusive?

Query: Hi doctor, I am a 28 year old male and had unprotected anal-vaginal intercourse with a sex worker, me being the active partner. I was put on PEP immediately within 11 hours post exposure and the first dose of PEP was the tablet TLE (Tenofovir or Lamuvidine or Efavirenz), given to me on the morning o...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have noted your concern. Perhaps your question was not completely typed. Anyways, your PEP regime of Tenofovir/Emtricitabine and Dolutegravir is recommended one. Continue with it for 28 days. As far as the HIV PCR RNA test is concerned it is not a reliable test w...  Read Full »

My HIV test came back negative at eight weeks. Am I all clear?

Query: Hello doctor, I would like some advice, please regarding HIV testing. 10 days following a sexual encounter, (protected penetrative sex, unprotected oral sex) I was tested at my local GUM clinic. Although the lady from my encounter tested negative, mine came back as weakly reactive (fourth generation...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have noted your concern. After an indeterminate or weakly positive HIV screening test (usually due to cross-reacting antibodies), the second confirmatory test should be a different yet more specific test, such as a western blot. Western blot is 99.9 % specific, wh...  Read Full »

Is the CIMA test a confirmatory test for HIV?

Query: Hello doctor,I had unprotected vaginal sex with an unknown girl for two minutes. I have taken the following tests: ECLIA third generation after 17 days of exposure, ECLIA third generation after 30 days of exposure, RNA by PCR after 31 days of exposure, TRI-DOT test after 50 days of exposure, and CMI...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. A 12-week screening test for HIV (human immunodeficiency virus) antibodies is considered conclusive, and a CMIA (chemiluminescent microparticle immunoassay) fourth generation test at 59 days of exposure with a window period of two to three weeks is very reliable, but...  Read Full »

How accurate is HIV-1 RNA, QL TMA?

Query: Hello doctor, Is HIV RNA, QL TMA accurate after 20 to 21 days of exposure? And also this came with my results. RNA is not detected. No laboratory evidence of HIV infection. The HIV-1 RNA qualitative TMA assay is recommended for use as part of a multi-test HIV-1/HIV-2 screening and diagnostic algori...  Read Full »


Dr. Sushil Kakkar

Answer: Hi, Welcome to icliniq.com. HIV RNA PCR test is accurate after 9 to 13 days after the risk exposure. It can be used to detect early infection with HIV when most other screening tests are not yet reliable. However, it is not a confirmatory test and an early screening with RNA PCR should be followed...  Read Full »

How accurate is the HIV test at 11 weeks?

Query: Hello doctor,I had cunnilingus with an escort. After this encounter, I had an RNA PCR test at 15 days and anti-HIV 1 and 2 as well as p24 antigen test after two, four, six, eight and 11 weeks. All the results were negative. But, while some websites say that the result is conclusive after eight wee...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have gone through your query and have noted your concern. HIV (human immunodeficiency virus) antibody screening test is highly sensitive at four weeks and thereafter. It accurately detects a majority (95 % sensitivity) of HIV-infected patients. However, in case of a ...  Read Full »

Are ELFA and HIV DUO tests conclusive at three and six months post-exposure?

Query: Hello doctor, I have had a few unprotected high-risk exposure in my life so far. I had a concern and checked my status of HIV through an ELFA test after three months of the last encounter. I did another ELFA test after six months, and both were non-reactive. I did not have any exposure in between t...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have gone through your query, and I have noted your concern. Your last screening tests for HIV (human immunodeficiency virus), that is, fourth generation p24 antigen and anti-HIV antibody screening test is conclusive for all previous exposures. Negative antibody scr...  Read Full »

Is a negative HIV screening test at 36 days conclusive?

Query: Hello doctor,I had a low-risk event (no penetration but exchange of fluids through fingering and immediate masturbation). At 26 days, I received a PCR/RNA test that came back undetectable. At 30 days, I felt a small posterior cervical lymph node which I know is connected to HIV. At 36 days, I receiv...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have noted your concern. A fourth-generation HIV screening test (antigen /antibody) is considered conclusive at four weeks. So, a negative test at 36 days is conclusive. Regards. For more information consult an HIV AIDS specialist online --> https://www.icliniq...  Read Full »

I tested negative for HIV twice. Can I be a case of late seroconversion?

Query: Dear doctor, Under a lot of alcohol, I had vaginal intercourse with a lady. I was wasted but I remember that I was protected with a condom. The condom did not break nor leak but maybe was not put perfectly. I did not manage to cum, removed it and masturbated in a very aggressive way with my bare ha...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have noted your concern. Protected sex is safe as far as the risk of HIV is concerned. Spitting on bare penis is not a risk for HIV as saliva is not infectious. The virus cannot survive in saliva because it contains enzymes inhibitory for the virus. Moreover, a f...  Read Full »

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