Q. Got two negative RNA TMA test for HIV. Are the results conclusive?

Answered by
Dr. Jyotisterna Mittal
and medically reviewed by iCliniq medical review team.
Published on Jun 15, 2016 and last reviewed on: Aug 07, 2019

Hello doctor,

I had two negative Gen-Probe Aptima RNA TMA test done the on the 8th day and 14th day of post exposure. I took a Duo Combo test on the 29th day. Has anyone seen these RNA tests after 14 days have become wrong?

Dr. Jyotisterna Mittal

Cosmetology Dermatology HIV/AIDS Specialist Venereology
#

Hi,

Welcome to icliniq.com.

  • You need not go for further testing as these tests are conclusive.

For further information consult a HIV AIDS specialist online --> https://www.icliniq.com/ask-a-doctor-online/HIV-AIDS-specialist

Thank you doctor,

Are the two RNA tests conclusive?

Dr. Jyotisterna Mittal

Cosmetology Dermatology HIV/AIDS Specialist Venereology
#

Hi,

Welcome back to icliniq.com.

  • You have undergone two RNA PCR testing. They are very accurate and pick up even a few copies of HIV (human immunodeficiency virus) from the blood within 3 days to 4 weeks after exposure.
  • This test is most useful in monitoring the viral load, once HIV is established.
  • However, this test is not used as a screening test for HIV infection, as there can be false positive and false negative results sometimes.
  • You have also taken a Duo Combo test on the 29th day. This test is the most useful test for screening after possible exposure.
  • Modern day combo can pick up infection from 2 to 4 weeks after exposure and are extremely sensitive.
  • A negative test means there are 99.7% chances that you are HIV free. Nothing is absolute in medicine and so 100% absolute surety is not there.
  • Still, if you want to be double sure, you may go for a repeat Duo Combo test after 12 weeks of possible exposure.
  • If it again comes negative, then you can sit back and relax that you do not have HIV.

For further information consult a HIV AIDS specialist online --> https://www.icliniq.com/ask-a-doctor-online/HIV-AIDS-specialist

Thank you doctor,

I am still waiting for the 4th Gen-Probe and Duo Combo results.

Dr. Jyotisterna Mittal

Cosmetology Dermatology HIV/AIDS Specialist Venereology
#

Hi,

Welcome back to icliniq.com.

  • In that case, you can expect a positive result for you.
  • If the enzyme-linked immunosorbent assay (ELISA) test is negative, you may follow the above said instructions.
  • If the test is positive, it must be followed up with a western blot test to confirm positivity as ELISA may be false positive in 5% of samples.
  • RNA PCR is not used for acute exposure screening.
  • A negative RNA PCR test may give us some assurance that chances of HIV are lesser, but it should always be followed by ELISA tests, because of the lower accuracy of PCR for screening purpose.

For further information consult a HIV AIDS specialist online --> https://www.icliniq.com/ask-a-doctor-online/HIV-AIDS-specialist

Thank you doctor,

I got RNA TMA (NAAT) and not a PCR. Can I rely on the report?

Dr. Jyotisterna Mittal

Cosmetology Dermatology HIV/AIDS Specialist Venereology
#

Hi,

Welcome back to icliniq.com.

I can understand your concern and apprehension.

  • Aptima RNA TMA and nucleic acid amplification test - NAAT are an improved version of the standard RNA PCR testing.
  • While the accuracy is higher than the conventional PCR, but the bottom line facts remain as such. That is why it is an aid in diagnosing along with HIV ELISA testing and cannot replace ELISA as a screening test.
  • Though some studies report it as a good alternative for western blot test, but sensitivity is low, so ELISA is still needed.
  • Moreover, it is not Food and Drug Administration (FDA) approved for screening.
  • HIV testing is all about probabilities and hence a good time tested combination of the tests should be taken as confirmatory.

For further information consult a HIV AIDS specialist online --> https://www.icliniq.com/ask-a-doctor-online/HIV-AIDS-specialist

Thank you doctor,

The two negative tests are highly encouraging right?

Dr. Jyotisterna Mittal

Cosmetology Dermatology HIV/AIDS Specialist Venereology
#

Hi,

Welcome back to icliniq.com.

  • Yes, you are right. They are highly encouraged and assuring that your ELISA combo test should also most probably will come out as negative.

For further information consult a HIV AIDS specialist online --> https://www.icliniq.com/ask-a-doctor-online/HIV-AIDS-specialist

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