Common "Enzyme Immunoassay" queries answered by top doctors | iCliniq

Enzyme Immunoassay

An enzyme-linked immunoassay is a diagnostic tool used to measure antibodies, antigens, proteins, and glycoproteins in biological samples. Some examples include diagnosis of HIV infection, pregnancy tests, and measurement of cytokines or soluble receptors in cell supernatant or serum. Enzyme-immune assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane. Enzyme-linked immunoassay uses an antibody conjugated to an enzyme (alkaline phosphatase and horseradish peroxidase) to bind the antigen. The enzyme converts a substrate (usually either chromogen or fluorogen) into an observable end product.

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I tested for HSV six weeks after the possible exposure, and the results were negative. Should I go for a retest?

Query: Hello doctor, I had a test on day-42 (exact six weeks) post a possible exposure. The result shows Herpes simplex virus (HSV) 1 and 2 IgM antibody (Serum, (Enzyme Immunoassay) EIA). Result: Negative (0.64). Associated tests were HSV 1 and 2, western blot IgM and IgG. 1. What exactly is this test loo...  Read Full »

Dr. G. S. S. Sandeep

Answer: Hello, Welcome to Enzyme Immuno Assay (EIA) is a type of ELISA (enzyme-linked immunosorbent assay). The test usually does not give positive results until about six weeks after exposure. The gold standard test would be a viral culture. If that is not available then, a western blot is re...  Read Full »

How likely am I to infect people with C. difficle while at work?

Query: Hello doctor, How likely is it that I might unwittingly cause people to be infected/ colonized with some harmful microbe (for example, C. difficile) while at work? I have been having some serious anxiety issues from thinking and worrying about this. I have been working in a restaurant for the past o...  Read Full »

Dr. Mahmoud Ahmed Abdelrahman Abouibrahim

Answer: Hi, Welcome to Clostridium difficile infection is of two types: 1. Symptomatic (causing diarrhea mainly): which is either due to transmission through infected food and contaminated hands or after antibiotic therapy. 2. Asymptomatic carrier (like your case). You have to perform clos...  Read Full »

I am worried about my lab results. Is it an emergency to consult a rheumatologist?

Query: Hello doctor, I am worried about my blood work lab results. My RNP antibodies are at 2.3, speckled pattern 1:80, antinuclear antibodies, IFA is positive. I know I should not be diagnosing myself, but my findings is worrisome. Most of my symptoms match up with what I am seeing. I guess my question i...  Read Full »

Dr. Anshul Varshney

Answer: Hello, Welcome to Based on the details available, it looks that you have lupus. Possibility of mixed connective tissue disorder (MCTD) is also there. You need furthest tests that include Anti-ds DNA, rheumatoid factor. At this point, you should not get worried unnecessarily. Wait for ...  Read Full »

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