Hplc

HPLC, otherwise called high-performance liquid chromatography, is a method used to identify, separate, and measure all the components present in a mixture. It is used to detect the vitamin D levels in the blood, detect performance-enhancing drugs in urine, and during the production of pharmaceutical products.

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What precaution should I take to conceive if both the partners have beta thalassemia?

Query: Hello doctor, My HBA2 is 5.8 (beta-thalassemia trait) and my husband HBA2 is 4. We have a daughter whose HBA2 is 3.6 (delta beta thalassemia trait). We do not have any issues still now. We are planning for the second issue. Please suggest. What test required and how much safety is there?  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hello, Welcome to icliniq.com. You are certainly having beta thalassemia minor. But your husband's HBA2 value is borderline. So to confirm the diagnosis of beta thalassemia minor of him by CBC (complete blood count), peripheral smear examination report, and genetic study in the good laboratory. ...  Read Full »

Can constipation be caused by iron tablets?

Query: Hi doctor, My wife who is 40 years old has hemoglobin level of 8. Which iron or other supplement should she take immediately? She regularly feels quite tired. She had the same issues during her college days too. Also, can iron tablets cause constipation? If yes, kindly suggest remedial measures. Pl...  Read Full »


Dr. Dattaprasad Balasaheb Inamdar

Infertility Specialist, Obstetrician And Gynaecologist

Answer: Hi, Welcome to icliniq.com. If she is having chronic anemia then one advice would be to make sure that it is due to iron deficiency anemia indeed and not due to other deficiency or thalassemia minor. If hemogram was done, red blood cell distribution width RDW of ≥15% and picture of micro...  Read Full »

Is marrying a partner with same blood group harmful?

Query: Hello doctor, Blood group of the male is A positive and blood group of the female is A with Rh positive. Can they get married? Will they face any problem in getting pregnant?  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hi, Welcome to icliniq.com. There is no harm in marrying the partner of same blood group. Every individual has unique own blood group and ABO and Rh system used most commonly. If you want to do preconception counseling then investigate with CBC (complete blood count) and if needed HPLC (high-per...  Read Full »

Can couple with same blood group give birth to healthy child?

Query: Hello doctor, My brother is worried about his marriage because of the same blood group. Both are having a B+ blood group. Is there any problem after marriage for getting a healthy baby? Please explain it to me so that I can talk to him.  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hello, Welcome to icliniq.com. Same blood group people can marry and can produce a normal child. There is two common universal blood group system that is ABO and Rh. If they both are relative then genetic counselling is needed. As far as same blood group is concerned, do not worry about that. If y...  Read Full »

Can you please go through my electrophoresis report and tell me if I have thalassemia minor?

Query: Hi doctor, Could you please go through my reports and tell me if I have thalassemia minor? I have attached my electrophoresis test reports. Some of the values in the report are Hb F - 0.8 %, Hb adult - 66.0 %, Hb A2 - 30.8 % and other non-specific - 2.4 %.  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hi, Welcome to icliniq.com. According to the report attached (attachment removed to protect patient identity), the Mentzer index is not below 13. Usually, in thalassemia, it is below 13. Hb A2 (hemoglobin A2) level is raised, which is seen in beta thalassemia minor as well as in hemoglobin E hemogl...  Read Full »

Can you see the attached CBC report, and let me know if there is anything serious ?

Query: Hi doctor, My wife's iron and ferritin were low, so the doctor ordered CBC. CBC showed low WBC, high monocytes, and high RBC, which is what made us scared. She has schistocytes and elliptocytes among other RBC morphologies. We read about it and are very worried, and she had a C-section three years...  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hello, Welcome to icliniq.com. I have gone through your wife's reports (attachment removed to protect patient identity). The report shows schistocytes, which are fragmented RBC (red blood cell), commonly seen in hemolytic anemia. So, to rule out hemolysis, serum LDH (lactate dehydrogenase), ret...  Read Full »

Will same blood group of husband and wife cause problem in pregnancy?

Query: Hello doctor,I and my husband belong to A+ blood group. Is there any problem to get pregnant? And I may attain my periods one week before the date. Is this good for me?  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hello, Welcome to icliniq.com. The same blood group people can marry and produce a normal child. As far as same blood group people are concerned, do not worry about that. Rule out hemoglobinopathy before marriage by Hb electrophoresis by HPLC (high-performance liquid chromatography) method. If you...  Read Full »

Will marrying a girl of same blood group create any problems in having children?

Query: Hi doctor, I have A+ blood group. But, will marrying a girl of the same blood group (A+) create any problems in having children or they lead to a diseased child?  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hello, Welcome to icliniq.com. The same blood group people can marry and can produce a normal child. Check your both thalassemia minor report before marriage by HB electrophoresis, by HPLC (high-performance liquid chromatography) method that is necessary. If you both are relative, then genetic cou...  Read Full »

Is it advisable for B-ve girl to have a baby with O+ve boy?

Query: Hello doctor, My girlfriend is B- group and I am O+ group. Is it advisable for us to get married or give birth?  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hello, Welcome to icliniq.com. Yes, the blood group is not at all barrier in your marriage. Your wife is Rh negative, so will be given anti-D immunoglobulin injection during pregnancy at 28th and 34th week of gestation to prevent hemolytic disease of the newborn. So do not worry about that. I will...  Read Full »

Kindly review my blood report and explain the type of thalassemia.

Query: Hi doctor,I have attached a report. I just want to know if this hemoglobin electrophoresis result is beta thalassemia minor or beta thalassemia intermedia or beta thalassemia major.  Read Full »


Dr. Parth R Goswami

Family Physician, General Practitioner, Hematologist, Pathologist

Answer: Hi, Welcome to icliniq.com. According to HPLC report (high-performance liquid chromatography), you have attached, HbA2 raised and HbA decreased (hemoglobin A2 and A). Hence, it is suggestive towards thalassemia minor (attachment removed to protect patient identity). It does not look like thalassemi...  Read Full »

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Disclaimer: All health Q&As published on this website are not intended to be a substitute for professional medical diagnosis, advice or treatment by a trained physician. Seek the advice from your physician or other qualified health-care providers with questions you may have regarding your symptoms and medical condition for a complete medical diagnosis. Do not delay or disregard seeking professional medical advice because of something you have read on this website.