Western Blot Test

The Western blot test is used to diagnose HIV (human immunodeficiency virus) infection. The collected blood sample is tested for the presence of HIV antibodies produced by the body to the virus and not the virus itself. This test is used along with the ELISA test to confirm HIV infection.

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I have positive IgG HSV-2 on a blood test but negative on the western blot test. Kindly help.

Query: Hi doctor,Six months before, I ordered an STD panel and received a high level of herpes-2 on the IgG test. I did a retest using IgG four days later and got a high result. Then about six weeks later, I sent in a sample for western blot, and it came back negative for types 1 and 2. My partner did the ...  Read Full »


Dr. N. Ashok Viswanath

Answer: Hi, Welcome to icliniq.com. I can understand your concern. Your herpes-2-IgG is positive, and your western blot test is negative in the report (attachments removed to protect the patient's identity). Since your western blot (gold standard serological test) came negative, we can take it as conclusive...  Read Full »

What kind of muscle pain is associated with ARS?

Query: Hi, I am a 31 year old male. About 38 days before I had sex with a CSW (Commercial Social Worker). It was protected vaginal sex and protected oral sex. Also there were deep throat kisses which lasted almost a minute. I am not sure if the condom was torn during the intercourse.  Since last 3 weeks ...  Read Full »


Dr. Sidharth Sonthalia

Answer: Hi,  Welcome to icliniq.com. Based upon your history, my answer to your questions: 1. Are My Symptoms Related to ARS? Acute Retroviral Syndrome (ARS) typically begins 7-14 days after the exposure to a HIV positive patient, and may last for up to 3-4 months. The symptoms are typically "flu-l...  Read Full »

Will HIV test be negative while on ART?

Query: Hi doctor, I am under ART for HIV. If I test for HIV now, will it be negative since I am under treatment?  Read Full »


Dr. Sidharth Sonthalia

Answer: Hello, Welcome to icliniq.com. Despite the currently available antiretroviral therapy (ART), your HIV (human immunodeficiency virus) detection tests like ELISA (enzyme-linked immunoassay) and western blot are not expected to become negative. There is no medically confirmed, published, or acknowled...  Read Full »

Are my flu-like symptoms due to HIV infection?

Query: Hello doctor, About 4 weeks ago, I got oral sex from a partner whose HIV status is unknown to me. I did only that with her. I always thought that it represented no risk of contamination to HIV. Now the thing is that about 1 week after that intercourse, I did a general blood test and found out that ...  Read Full »


Dr. Meha Malhotra

Answer: Hi, Welcome to icliniq.com. Firstly pre-existing lesion of herpes or any genital ulcer on the penis during sexual intercourse or oral sex increases the chances of HIV transmission. Your blood report came positive for Herpes 1 and 2. Herpes 1 mostly causes oral ulcers, and herpes 2 causes genital l...  Read Full »

Are hot breaths and acidity symptoms of an HIV infection?

Query: Hi doctor, I am having hot breaths, and my eyes feel hot. I have acidity problems. All these problems started after I had sexual intercourse. Is it an indication of HIV? Should I get a western blot test now?  Read Full »


Dr. Murali R

Answer: Hi, Welcome to icliniq.com. Hot breaths and acidity problems are non-specific symptoms and are not related to HIV (human immunodeficiency virus) infection. The risk factors for HIV infection is having sex with unknown partners, whose HIV status is not known, or through blood transmission. If you ha...  Read Full »

What are the tests done to detect HIV?

Query: Hello doctor, I had unprotected sex four weeks back. Later on, I cross checked with the girl, and she stated she do not have any disease. But still, I am worried. After three weeks I have checked with P24 antigen test, the test value is 0.0, and it is non-reactive. By the fourth week (28th day) I ha...  Read Full »


Dr. Atishay Bukharia

Answer: Hi,Welcome to icliniq.com.It could be false positive. You have more than 95 percent chances of being negative.Sore throat may or may not be a symptom of HIV.Go for western blot test and HIV RT-PCR (real-time polymerase chain reaction) test to confirm the status of HIV.For further information consul...  Read Full »

Is lymphadenopathy after unprotected oral sex a sign of HIV?

Query: Hi doctor, My issue is that I had unprotected oral sex with a CSW a few months before. Since then, I have fleeting lymphadenopathy till date. I have done my HIV tridot after a month, and it was negative. I had an HIV ELISA (enzyme-linked immunosorbent assay) test done in the same month after ten da...  Read Full »


Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to icliniq.com. 1) Unprotected oral sex carries a low risk of acquiring HIV, especially if there are bleeding gums or active oral ulcers. 2) If the last exposure was a few months ago and there had been no further exposure, your test results for HIV will be considered conclusive. The...  Read Full »

What test should I undergo to confirm my HIV status?

Query: Hi doctor, I am sexually active for the past three years, but I have never checked my HIV nor done any tests. But, I had sex with a sex worker with protection but still got scared it has been seven months. I did not have sex. What test do I need to do? Do I need to test? I used a condom, and I hear...  Read Full »


Dr. Ashok Kumar Choudhary

Answer: Hello, Welcome to icliniq.com. First of all, I like to inform you that getting HIV while using condom/protection is very low and almost negligible. In other words, it can be stated that the chance is almost nil. Regarding the test, I feel Elisa for HIV will be most suitable for you as the exposur...  Read Full »

Will certain medicines interfere with HIV test results?

Query: Hello doctor, Does Ashwagandha delay on HIV test? Does any medicine delay on HIV test? How accurate is the HIV test in six months with the fourth generation?  Read Full »


Dr. Penchilaprasad Kandikattu

Answer: Hello, Welcome to icliniq.com. Usually, Ashwagandha shows some beneficial results like neuroprotective effect or boosting immunity in HIV (human immunodeficiency virus) positive patients on a part of the treatment plan. But it is not going to delay any HIV test. Usually, after exposure to HIV ...  Read Full »

Can any undiagnosed underlying disease interrupt with the test result of HIV?

Query: Hello doctor, I had a possible exposure before November 2019. I do not know the exact date. It was oral sex after that I got tested on 19th April, May 28th, and June 14th 2020 all were rapid tests and negative and Elisa on 15th June and it was also negative. My question is, can any other underlyi...  Read Full »


Dr. Pathave Hari Shivaram

Answer: Hi, Welcome to icliniq.com. If you have tested three times April, May, and June and exposure is on November 19, then it indicates you are negative for HIV completely. You have done a rapid test and Elisa. Western blot test is more confirmatory. Yes, that false negative concept is there but if you...  Read Full »

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