Western Blot Test

The Western blot test is used to diagnose HIV (human immunodeficiency virus) infection. The collected blood sample is tested for the presence of HIV antibodies produced by the body to the virus and not the virus itself. This test is used along with the ELISA test to confirm HIV infection.

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All the answers published in this website are written by Verified medical doctors, therapists and health experts. The Content has been moderated by iCliniq medical review team before publication. Post your medical clarifications on iCliniq by choosing the right specialty and get them answered. Your medical queries will be answered 24/7 by top doctors from iCliniq.

What test should I undergo to confirm my HIV status?

Query: Hi doctor, I am sexually active for the past three years, but I have never checked my HIV nor done any tests. But, I had sex with a sex worker with protection but still got scared it has been seven months. I did not have sex. What test do I need to do? Do I need to test? I used a condom, and I hear...  Read Full »


Dr. Ashok Kumar Choudhary

Andrologist, General Practitioner, Geriatrician, Psychiatrist, Psychologist Counsellor, Psychotherapist, Sexologist

Answer: Hello, Welcome to icliniq.com. First of all, I like to inform you that getting HIV while using condom/protection is very low and almost negligible. In other words, it can be stated that the chance is almost nil. Regarding the test, I feel Elisa for HIV will be most suitable for you as the exposur...  Read Full »

Will certain medicines interfere with HIV test results?

Query: Hello doctor, Does Ashwagandha delay on HIV test? Does any medicine delay on HIV test? How accurate is the HIV test in six months with the fourth generation?  Read Full »


Dr. Penchala Prasad Kandikattu

General Medicine Physician, Internal Medicine Physician

Answer: Hello, Welcome to icliniq.com. Usually, Ashwagandha shows some beneficial results like neuroprotective effect or boosting immunity in HIV (human immunodeficiency virus) positive patients on a part of the treatment plan. But it is not going to delay any HIV test. Usually, after exposure to HIV ...  Read Full »

Can any undiagnosed underlying disease interrupt with the test result of HIV?

Query: Hello doctor, I had a possible exposure before November 2019. I do not know the exact date. It was oral sex after that I got tested on 19th April, May 28th, and June 14th 2020 all were rapid tests and negative and Elisa on 15th June and it was also negative. My question is, can any other underlyi...  Read Full »


Dr. Hari Pathave

Dermatologist, Venereologist

Answer: Hi, Welcome to icliniq.com. If you have tested three times April, May, and June and exposure is on November 19, then it indicates you are negative for HIV completely. You have done a rapid test and Elisa. Western blot test is more confirmatory. Yes, that false negative concept is there but if you...  Read Full »

What are the recommended detectable tests for HIV?

Query: Hello doctor, I did protected sex with a known lady two times, (65 days before and 36 days back). She has breast milk, I swallowed a couple of drops at that time. I felt fatigue, and a couple of bumps on the back of the neck after five days of second exposure. I got tested HIV duo combo after 28...  Read Full »


Dr. Noushin Payravi

Family Physician, General Practitioner

Answer: Hello, Welcome to icliniq.com. Thanks for sharing your medical info with us. In terms of HIV detection tests, ELISA (enzyme-linked immunosorbent assay test) is the first test to be done and then if positive, western blot is recommended. You and your partner have both tested negative with rapid H...  Read Full »

What kind of muscle pain is associated with ARS?

Query: Hi, I am a 31 year old male. About 38 days before I had sex with a CSW (Commercial Social Worker). It was protected vaginal sex and protected oral sex. Also there were deep throat kisses which lasted almost a minute. I am not sure if the condom was torn during the intercourse.  Since last 3 weeks ...  Read Full »


Dr. Sidharth Sonthalia

Cosmetologist, Dermatologist, Hair Transplant Surgeon, HIV AIDS Specialist, Venereologist

Answer: Hi,  Welcome to icliniq.com. Based upon your history, my answer to your questions: 1. Are My Symptoms Related to ARS? Acute Retroviral Syndrome (ARS) typically begins 7-14 days after the exposure to a HIV positive patient, and may last for up to 3-4 months. The symptoms are typically "flu-l...  Read Full »

Is CMIA test accurate?

Query: Hello doctor, My doctor has recommended western blot for HIV-1 and HIV-2 as a screening test. The RIBA test was negative for HIV, after 16 weeks of exposure. I also had a negative CMIA test, after 12 weeks post exposure. Hepatitis and STD panel are also negative. Is a negative western blot 100 % ac...  Read Full »


Dr. Ravinder Kaur Sachdeva

HIV AIDS Specialist

Answer: Hi, Welcome to icliniq.com. Western blot is highly sensitive and specific test, which is run after a repeatedly reactive ELISA or HIV (human immunodeficiency virus) rapid test. Western blot test is not used as a diagnostic test in the absence of an initial positive screening test, due to the rar...  Read Full »

How accurate is HIV 1 RNA test?

Query: Hello doctor, I have done RNA PCR test. The report showed that the target was not detected in the collected serum. HIV RNA was detected below, within and above the linear range of the assay. Can we conclude that the virus has entered into the body? After one week, I got western blot test also done....  Read Full »


Dr. Rakesh Kumar Bahunuthula

Cosmetologist, Dermatologist, HIV AIDS Specialist, Venereologist

Answer: Hi, Welcome to icliniq.com. Kindly mention the kind of exposure you had, whether it was protected, the duration since the exposure and the timing of the test you have undergone with respect to the exposure. Are you experiencing any symptoms since the exposure? Western blot is a second line test a...  Read Full »

What are the ways to confirm HIV?

Query: Hi doctor, I had unprotected anal and oral sex with a guy. This makes me very stressful. The symptoms I encounter after the incident are as follows. I am feeling very tired from day 10, and I think it as stress. From day 12, I am having some sort of difficulty in breathing and numbness all over the ...  Read Full »


Dr. Sidharth Sonthalia

Cosmetologist, Dermatologist, Hair Transplant Surgeon, HIV AIDS Specialist, Venereologist

Answer: Hi, Welcome to icliniq.com. Thanks for providing the clinical pictures (attachment removed to protect patient identity). I would try to provide you with the best possible answers to your questions. The symptoms you have mentioned for example fever, general feeling of not being well, vague body ac...  Read Full »

Are my flu-like symptoms due to HIV infection?

Query: Hello doctor, About 4 weeks ago, I got oral sex from a partner whose HIV status is unknown to me. I did only that with her. I always thought that it represented no risk of contamination to HIV. Now the thing is that about 1 week after that intercourse, I did a general blood test and found out that ...  Read Full »


Dr. Meha Malhotra

Dermatologist, HIV AIDS Specialist, Venereologist

Answer: Hi, Welcome to icliniq.com. Firstly pre-existing lesion of herpes or any genital ulcer on the penis during sexual intercourse or oral sex increases the chances of HIV transmission. Your blood report came positive for Herpes 1 and 2. Herpes 1 mostly causes oral ulcers, and herpes 2 causes genital l...  Read Full »

Does HSV positivity indicate risk of HIV?

Query: Hello doctor, I am a 28 year old male. I had an exposure with a CSW and precaution broke during the act. I do not know the HIV status of the girl. Four weeks later, I got a light fever which subsided in a week. I got a blood test that showed 11500 WBC with a bacterial infection. But, I have leg pai...  Read Full »


Dr. Sidharth Sonthalia

Cosmetologist, Dermatologist, Hair Transplant Surgeon, HIV AIDS Specialist, Venereologist

Answer: Hello, Welcome to icliniq.com. First of all, do not panic. Your results till now are highly suggestive (but not 100 % confirmatory) of you being HIV (human immunodeficiency virus) negative. Please note: Getting triple ELISA (enzyme-linked immunosorbent assay) with p24 Ag test every few days will not...  Read Full »

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