Western Blot Test

The Western blot test is used to diagnose HIV (human immunodeficiency virus) infection. The collected blood sample is tested for the presence of HIV antibodies produced by the body to the virus and not the virus itself. This test is used along with the ELISA test to confirm HIV infection.

Recently Answered Questions Recently Answered Questions

All the answers published in this website are written by Verified medical doctors, therapists and health experts. The Content has been moderated by iCliniq medical review team before publication. Post your medical clarifications on iCliniq by choosing the right specialty and get them answered. Your medical queries will be answered 24/7 by top doctors from iCliniq.

My HIV test came back negative at eight weeks. Am I all clear?

Query: Hello doctor, I would like some advice, please regarding HIV testing. 10 days following a sexual encounter, (protected penetrative sex, unprotected oral sex) I was tested at my local GUM clinic. Although the lady from my encounter tested negative, mine came back as weakly reactive (fourth generation...  Read Full »


Dr. Sushil Kakkar

Answer: Hello, Welcome to icliniq.com. I have noted your concern. After an indeterminate or weakly positive HIV screening test (usually due to cross-reacting antibodies), the second confirmatory test should be a different yet more specific test, such as a western blot. Western blot is 99.9 % specific, wh...  Read Full »

I have been tested negative for HIV and western blot test. How accurate are those?

Query: Hello doctor,How accurate are HIV tests done in the laboratory on the 56th day of exposure along with the western blot test? Both tests came negative. I took the flu vaccine 18 days before the test and had no symptoms except minor rashes. Kindly help.  Read Full »


Dr. Rajesh Jain

Answer: Hello, Welcome to icliniq.com. Thank you for your query. There is nothing to worry as your tests are negative, and you are free from HIV (human immunodeficiency virus). I hope this has helped you. Thank you.  Read Full »

Sixty days after possible exposure, I got tested for HIV, and the results came negative. Why is the serum control band present in the western blot test?

Query: Hello doctor, I have taken an HIV test due to a recent possible exposure. The incident happened 60 days ago. So I did a rapid HIV test. It is negative. I also did a western blot test. HIV 1 and HIV 2 are negative, but the serum control band is present. What does it mean? Is it indicative of an HIV i...  Read Full »


Dr. Basti Bharatesh Devendra

Answer: Hello, Welcome to icliniq.com. It is good that the HIV rapid and western blot tests are negative after 60 days of exposure. The chances of HIV tests coming positive later are almost negligible. But it is better to go for an HIV antibodies test after three months of exposure in a reliable laborator...  Read Full »

I have undergone a wide range of HIV tests, all of which were negative except the Western blot test, which was indeterminate. What does that conclude?

Query: Hello doctor, I have undergone a wide range of HIV testing. The test values are - 1) On 78, 88, 96, 110, 120, 126, 158, 164, and 168th day, the fourth generation antigen and antibody tests were all negative, and on the 32nd and 64th days, HIV rapid test was negative. 2) HIV 1 DNA PCR qualitative on ...  Read Full »


Dr. Rakesh Kumar Bahunuthula

Answer: Hi, Welcome to icliniq.com. I have read your query and understand your concern. As the tests beyond 12 weeks are negative, you can consider yourself not to have acquired the infection. Indeterminate result in Western blot is a false positive as it does not correspond with the other test results. T...  Read Full »

I have positive IgG HSV-2 on a blood test but negative on the western blot test. Kindly help.

Query: Hi doctor,Six months before, I ordered an STD panel and received a high level of herpes-2 on the IgG test. I did a retest using IgG four days later and got a high result. Then about six weeks later, I sent in a sample for western blot, and it came back negative for types 1 and 2. My partner did the ...  Read Full »


Dr. N. Ashok Viswanath

Answer: Hi, Welcome to icliniq.com. I can understand your concern. Your herpes-2-IgG is positive, and your western blot test is negative in the report (attachments removed to protect the patient's identity). Since your western blot (gold standard serological test) came negative, we can take it as conclusive...  Read Full »

What kind of muscle pain is associated with ARS?

Query: Hi, I am a 31 year old male. About 38 days before I had sex with a CSW (Commercial Social Worker). It was protected vaginal sex and protected oral sex. Also there were deep throat kisses which lasted almost a minute. I am not sure if the condom was torn during the intercourse.  Since last 3 weeks ...  Read Full »


Dr. Sidharth Sonthalia

Answer: Hi,  Welcome to icliniq.com. Based upon your history, my answer to your questions: 1. Are My Symptoms Related to ARS? Acute Retroviral Syndrome (ARS) typically begins 7-14 days after the exposure to a HIV positive patient, and may last for up to 3-4 months. The symptoms are typically "flu-l...  Read Full »

Will HIV test be negative while on ART?

Query: Hi doctor, I am under ART for HIV. If I test for HIV now, will it be negative since I am under treatment?  Read Full »


Dr. Sidharth Sonthalia

Answer: Hello, Welcome to icliniq.com. Despite the currently available antiretroviral therapy (ART), your HIV (human immunodeficiency virus) detection tests like ELISA (enzyme-linked immunoassay) and western blot are not expected to become negative. There is no medically confirmed, published, or acknowled...  Read Full »

Are my flu-like symptoms due to HIV infection?

Query: Hello doctor, About 4 weeks ago, I got oral sex from a partner whose HIV status is unknown to me. I did only that with her. I always thought that it represented no risk of contamination to HIV. Now the thing is that about 1 week after that intercourse, I did a general blood test and found out that ...  Read Full »


Dr. Meha Malhotra

Answer: Hi, Welcome to icliniq.com. Firstly pre-existing lesion of herpes or any genital ulcer on the penis during sexual intercourse or oral sex increases the chances of HIV transmission. Your blood report came positive for Herpes 1 and 2. Herpes 1 mostly causes oral ulcers, and herpes 2 causes genital l...  Read Full »

Are hot breaths and acidity symptoms of an HIV infection?

Query: Hi doctor, I am having hot breaths, and my eyes feel hot. I have acidity problems. All these problems started after I had sexual intercourse. Is it an indication of HIV? Should I get a western blot test now?  Read Full »


Dr. Murali Rayani

Answer: Hi, Welcome to icliniq.com. Hot breaths and acidity problems are non-specific symptoms and are not related to HIV (human immunodeficiency virus) infection. The risk factors for HIV infection is having sex with unknown partners, whose HIV status is not known, or through blood transmission. If you ha...  Read Full »

What are the tests done to detect HIV?

Query: Hello doctor, I had unprotected sex four weeks back. Later on, I cross checked with the girl, and she stated she do not have any disease. But still, I am worried. After three weeks I have checked with P24 antigen test, the test value is 0.0, and it is non-reactive. By the fourth week (28th day) I ha...  Read Full »


Dr. Atishay Bukharia

Answer: Hi,Welcome to icliniq.com.It could be false positive. You have more than 95 percent chances of being negative.Sore throat may or may not be a symptom of HIV.Go for western blot test and HIV RT-PCR (real-time polymerase chain reaction) test to confirm the status of HIV.For further information consul...  Read Full »

ask-doctor-img Ask a Doctor Online

* Your first query consult is free!

Search for a Health Issue



Disclaimer: No content published on this website is intended to be a substitute for professional medical diagnosis, advice or treatment by a trained physician. Seek advice from your physician or other qualified healthcare providers with questions you may have regarding your symptoms and medical condition for a complete medical diagnosis. Do not delay or disregard seeking professional medical advice because of something you have read on this website. Read our Editorial Process to know how we create content for health articles and queries.