Thank you for the reply, This is context to my fist question to you, I know i'm panicking a lot over this, but I need to know certain answers to clear out my mind. My question to you was:
I have studied that HLA B-35, B-58, B-07 are responsible for rapid progression to AIDS and often test false negative when tested. However with C clade virus. It is some what protective and does not lead to rapid disease progression which has something to do with CD8 T cell response to a single GAG epitope which is only available in C clade virus. I want to know, Will a HLA B-35/58 person infected with C clade virus produce antibodies to HIV unlike in B clade virus and what causes false negative in B clade. To which your reply was: yes, HLA B35/58 person will be able to produce antibodies against HIV 1. This is true for all clades. The immunogenic response is known to be somewhat greater in clade C, due to higher diversity of immunogenic epitopes on p17 and p24 GAG protein. Clade B is known to be less diverse, leading to lower CD8 response and also due to presence of escape mutant at a higher frequency. A completely negative serology is extremely rare phenomenon. Some clade specific differences in p17-GAG epitope result in nonimmunogenicity in clade B. however, only few case reports of non reactive serology are known and are not often seen. My question is:Clade B is known to be less diverse, leading to lower CD8 response and also due to presence of escape mutant at a higher frequency. A completely negative serology is extremely rare phenomenon. 1) Will these people have rapid progression to AIDS? or is there a possibility that they do not progress to AIDS rapidly but might test negative for HIV in rare cases? I have done both a 4th generation and a western blot test-both the results are negative. 2) Do they look for all subtypes of HIV A,B,C,D,E,F,G,H,I,J,K and the CRF's of the following, can these tests miss out any crf combination between A-K, other than missiong out N and O group which I cannot have?