Q. Is ELISA test reliable?

Answered by
Dr. Atishay Bukharia
and medically reviewed by iCliniq medical review team.
This is a premium question & answer published on Jun 05, 2016 and last reviewed on: Apr 06, 2020

Hi doctor,

I am 29 years old. I had protected sex with a call girl before five months. But, I want to confirm whether I was infected with any disease. I have read on the internet that window period for HIV is three months and hence to confirm I have undergone HIV testing called ELISA. The result of which is non-reactive and the value is 0.062. Again, now I repeated the test and it showed result as non-reactive with the value of 0.104. The two reports have been attached here. Regarding symptoms, I did not develop any fever or allergy in the last four months. Is ELISA test reliable? Is there any need for me to take any other test or repeat the test? Can I stop worrying as I have already covered window period? Why did the test show some value like 0.6 and 0.104? Does it indicate the presence of any disease? What is the window period? Please help me in detail as I am much worried.

Dr. Atishay Bukharia

Cosmetology Dermatology HIV/AIDS Specialist Venereology
#

Hi,

Welcome to icliniq.com.

Let me answer one by one.

  • Yes, ELISA is highly reliable (enzyme-linked immunosorbent assay). It has got the sensitivity of 99.97% after three months of exposure, which means out of 10,000 HIV positive people it can rightly level 9,997 people as HIV positive.
  • There is no need to repeat any test or opt for further test. You can consider it as confirmatory.
  • Testing at six months is only recommended for those who has taken post-exposure prophylaxis with 72 hours of HIV exposure.
  • There is no need to worry. If the condom was intact during sex, then you are not even at risk.
  • Values does not matter as long as you are non-reactive and no two values of any test can be exactly the same, all have some technical limitations.
  • Window period is four weeks in more than 95% infected people and for remaining 5% it can extend up to three months. 
  • If you still want to get tested, then go for HIV RT-PCR (reverse transcriptase - polymerase chain reaction). It will detect any HIV virus element if present, but in my personal opinion you do not need this.

For further information consult a HIV AIDS specialist online --> https://www.icliniq.com/ask-a-doctor-online/HIV-AIDS-specialist


Was this answer helpful?

 | 

Also Read Answers From:

Comprehensive Medical Second Opinion.Submit your Case

Related Questions & Answers


What is the cut-off value for ELISA test?

Query: Hello doctor, I have some queries about HIV, ELISA/ECLIA TEST. What is the cut-off for this test? In some lab reports, they do not mention the cut off rate. They just mention if it is reactive or non-reactive. In some labs, 0.9 is the cut off while in others, it is different. Why is it so?  Read Full »

How is the CMIA combo test done for HIV testing?

Query: Hello doctor, I shall be thankful to you if you please answer few of my queries. How CMIA combo for HIV testing is done? How much it is reliable at 175 and 180 post incidence. INDEX was .14 and .08, respectively. What are the chances of error in the result? (Done at two different labs). Even after ...  Read Full »

I am under PEP. How often should I get tested for HIV if the results come negative?

Query: Hi doctor, I am 32 years old. I am on PEP that has to be taken for 28 days. I got my first dose around 40 hours of being contracted to HIV. PEP is not 100 % secure. My question is if I am lucky and the result shows HIV negative after the therapy, how often do I need to get antibody tested if luck s...  Read Full »

Popular

Popular

Ask your health query to a doctor online?

Ask a HIV AIDS Specialist Now

* guaranteed answer within 4 hours.
Enter Your Health Query
You can upload files and images in the next step.

Fee:  

 


Disclaimer: All health Q&As published on this website is not intended to be a substitute for professional medical diagnosis, advice or treatment by a trained physician. Seek the advice from your physician or other qualified health-care providers with questions you may have regarding your symptoms and medical condition for a complete medical diagnosis. Do not delay or disregard seeking professional medical advice because of something you have read on this website.