HomeAnswersHIV/AIDS specialisthiv testingShould I repeat the HIV tests after 28 days of exposure?

HIV 1 and 2 are nonreactive 28 days after exposure. Should I repeat it later?


The following is an actual conversation between an iCliniq user and a doctor that has been reviewed and published as a Premium Q&A.

Medically reviewed by

iCliniq medical review team

Published At November 19, 2017
Reviewed AtFebruary 12, 2024

Patient's Query

Hello doctor,

HIV (Human Immunodeficiency Virus) 1 and 2 are nonreactive in the tri-dot test done after 28 days of exposure. Should I repeat the test for confirmation?

Hello, Welcome to icliniq.com.

Tri-dot is a third-generation test for HIV (human immunodeficiency virus). HIV infection has a window period of one to three months, during which these test results may be false negative. According to WHO (world health organization), all the test results are considered conclusive 12 weeks after the last exposure. However, fourth-generation HIV tests are considered accurate one month after the last exposure. Hence, you can repeat the tri-dot after three months or go for a fourth-generation combo test one month after the last exposure to confirm the result.

Patient's Query

Thank you doctor,

HIV 1 RNA (Ribonucleicacid) RT (Reverse Transcriptase) PCR (Polymerase Chain Reaction) is also negative 14 days after exposure. Is this conclusive? Please help.


Welcome back to icliniq.com.

The test is almost 99 percent accurate by two weeks. However, as per WHO guidelines, we advise retesting after a baseline test.

Same symptoms don't mean you have the same problem. Consult a doctor now!

Dr. Rakesh Kumar Bahunuthula
Dr. Rakesh Kumar Bahunuthula


Community Banner Mobile
By subscribing, I agree to iCliniq's Terms & Privacy Policy.

Ask your health query to a doctor online

HIV/AIDS specialist

*guaranteed answer within 4 hours

Disclaimer: No content published on this website is intended to be a substitute for professional medical diagnosis, advice or treatment by a trained physician. Seek advice from your physician or other qualified healthcare providers with questions you may have regarding your symptoms and medical condition for a complete medical diagnosis. Do not delay or disregard seeking professional medical advice because of something you have read on this website. Read our Editorial Process to know how we create content for health articles and queries.

This website uses cookies to ensure you get the best experience on our website. iCliniq privacy policy