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Should I repeat an HIV test if the tridot test is negative?

This Premium Q&A, reviewed and published, features a real conversation between an iCliniq user and a physician.

Patient's Query

Hello doctor,

HIV (Human Immunodeficiency Virus) 1 and 2 are nonreactive in the tri-dot test done after 28 days of exposure. Should I repeat the test for confirmation?

Kindly help.

Answered by Dr. Rakesh Kumar Bahunuthula

Education:

MD

Professional Bio:

Dr. Rakesh Kumar Bahunuthula is a well-experienced Dermatologist, Cosmetologist, and Hair Transplant Surgeon. He is skilled in treating a wide range of skin, hair, and cosmetic concerns, including acne, pigmentation, aging-related changes, and skin disorders. Dr. Bahunuthula specializes in advanced hair restoration techniques, aesthetic procedures, laser treatments, and minimally invasive cosmetic solutions. He emphasizes personalized care, natural-looking results, and improving patients’ confidence and overall appearance.

This doctor is not available for online consultations on the platform anymore.

Hello,

Welcome to icliniq.com.

Tri-dot is a third-generation test for HIV (human immunodeficiency virus). HIV infection has a window period of one to three months, during which these test results may be false-negative. According to WHO (World Health Organization), all the test results are considered conclusive 12 weeks after the last exposure. However, fourth-generation HIV tests are considered accurate one month after the last exposure. Hence, you can repeat the tri-dot after three months or go for a fourth-generation combo test one month after the last exposure to confirm the result.

I hope this helps.

Patient's Query

Hi doctor,

HIV 1 RNA (Ribonucleicacid) RT (Reverse Transcriptase) PCR (Polymerase Chain Reaction) is also negative 14 days after exposure. Is this conclusive?

Please help.

Answered by Dr. Rakesh Kumar Bahunuthula

Education:

MD

Professional Bio:

Dr. Rakesh Kumar Bahunuthula is a well-experienced Dermatologist, Cosmetologist, and Hair Transplant Surgeon. He is skilled in treating a wide range of skin, hair, and cosmetic concerns, including acne, pigmentation, aging-related changes, and skin disorders. Dr. Bahunuthula specializes in advanced hair restoration techniques, aesthetic procedures, laser treatments, and minimally invasive cosmetic solutions. He emphasizes personalized care, natural-looking results, and improving patients’ confidence and overall appearance.

This doctor is not available for online consultations on the platform anymore.

Hello,

Welcome back to icliniq.com.

The test is almost 99 percent accurate by two weeks. However, as per WHO guidelines, we advise retesting after a baseline test.

I hope this helps.

Medically reviewed by iCliniq medical review team
Published At November 19, 2017
Reviewed At February 2, 2026

Education:

MD

Professional Bio:

Dr. Rakesh Kumar Bahunuthula is a well-experienced Dermatologist, Cosmetologist, and Hair Transplant Surgeon. He is skilled in treating a wide range of skin, hair, and cosmetic concerns, including acne, pigmentation, aging-related changes, and skin disorders. Dr. Bahunuthula specializes in advanced hair restoration techniques, aesthetic procedures, laser treatments, and minimally invasive cosmetic solutions. He emphasizes personalized care, natural-looking results, and improving patients’ confidence and overall appearance.

This doctor is not available for online consultations on the platform anymore.

Same symptoms don't mean you have the same problem. Consult a doctor now!

Education:

MD

Professional Bio:

Dr. Rakesh Kumar Bahunuthula is a well-experienced Dermatologist, Cosmetologist, and Hair Transplant Surgeon. He is skilled in treating a wide range of skin, hair, and cosmetic concerns, including acne, pigmentation, aging-related changes, and skin disorders. Dr. Bahunuthula specializes in advanced hair restoration techniques, aesthetic procedures, laser treatments, and minimally invasive cosmetic solutions. He emphasizes personalized care, natural-looking results, and improving patients’ confidence and overall appearance.

This doctor is not available for online consultations on the platform anymore.

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