Fourth Generation Hiv Test

HIV is a sexually transmitted disease which is caused by human immunodeficiency virus. There are various tests to detect the presence of HIV antigens/antibodies. These tests have evolved over time. A fourth-generation test identifies HIV antibodies as well as p24 antigens from the sample. Therefore, this test is more sensitive and helps identify the infection much earlier than a third generation test.

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Does random tenderness with swollen underarm lymphnode signify HIV infection?

Query: Hello doctor, I am 29-year-old, married male. I have a baby who feeds on breast milk. I had a sexual encounter before 8 months with a professional with a condom. I am 95 % sure that my protection was intact. Then I started reading about the HIV symptoms and technicalities of testing online. Five day...  Read Full »

Dr. Basti Bharatesh Devendra

Answer: Hi, Welcome to The chances of transmission of HIV (human immunodeficiency virus) by the given description is almost nil. HIV tests are repeatedly negative and after three months of exposure it is conclusive. You are free from HIV. There is no need for you to worry about seroconversion...  Read Full »

had a hand job with a massage girl after which I suffered from night sweats and pain in my lymph nodes. Can I get HIV?

Query: Hello doctor, I had a hand job with a massage girl a few months ago. I suffered from a burning sensation in my urine along with pain in my kidney and night sweats after that sexual activity. My HIV test came negative. But I am still having joint pain, night sweats, itching, and pain in the lymph nod...  Read Full »

Dr. Basti Bharatesh Devendra

Answer: Hello, Welcome to I went through your query and undersood your concern. The chances of transmission of HIV (human immunodeficiency virus) by single hand job is nil unless there are bleeding wounds in the hand. You would have noticed it. It is good that the HIV tests are negative. The ...  Read Full »

Why are my symptoms like flu and sore throat persisting despite getting negative fourth-generation tests multiple times after HIV exposure?

Query: Hello doctor,I was exposed to HIV some 15 weeks ago and I have gone for a fourth generation test at two weeks, four weeks, seven weeks, nine weeks, 11 weeks, and 14 weeks and a rapid test at 15 weeks and all came back negative. But I have some symptoms that are not going away like flu, sore throat, ...  Read Full »

Dr. Sidharth Sonthalia

Answer: Hello, Welcome to I suggest you calm down and do not panic. And while there are certain gaps in the information provided by you, allow me to first make and establish certain axiomatic deductions about your situation: 1) Presuming that your exposure to HIV (human immune virus) was non...  Read Full »

I had high-risk HIV exposure two years back, and all my HIV tests are negative. Is this conclusive?

Query: Hello doctor, After high-risk exposure to HIV, I had a negative 4th-generation combo and RNA at 14 days. Then a negative rapid Insti and rapid Alere Determine combo at 12 weeks. Also three negative rapid Insti and RNA after two years. Is this conclusive? Thank you.  Read Full »

Dr. Rakesh Kumar Bahunuthula

Answer: Hello,Welcome to have gone through your history and understand your anxiety and confusion.The fear of HIV (human immunodeficiency virus) after exposure is more dangerous, and it tends to persist in our minds for a very long time. Not everyone who has exposure acquires HIV. However, to...  Read Full »

Should I undergo any other screening tests for HIV apart from the HIV PCR RNA test ten weeks after exposure?

Query: Hello doctor, I had unprotected vaginal intercourse with a stranger four months ago. After four weeks, I observed rashes on my back on both sides and intense itching that persists till now. After ten weeks, I got tested for HIV PCR RNA test, and the result was undetected. After four months, I did th...  Read Full »

Dr. Shah Sushma Kant

Answer: Hello, Welcome to I have read your query and seen the attached pictures (the attachment is removed to protect the patient's identity). The rashes look like bruises in most places. The fourth-generation test is authentic and 99.9 % correct. Which means you are HIV-negative. For the s...  Read Full »

Is there a possibility that I am infected with HIV even if all the HIV antibody tests return negative?

Query: Hello doctor, It has been 22 months since my last exposure to HIV. I had done the TRI-DOT test, and comb AIDS test eight months after exposure to HIV, but they were negative. However, I have been having symptoms such as slight ear pain and sore throat for the past six months. And also, I get cold ea...  Read Full »

Dr. Basti Bharatesh Devendra

Answer: Hello, Welcome to Thank you for your query. I understand your concern. If the human immunodeficiency virus (HIV) antibodies test is negative after three months of last exposure, it is conclusive, provided there is no further risk exposure. The attached report (attachment removed to p...  Read Full »

I did a rapid test followed by a fourth-generation test after having protected sex, which came negative. Should I get tested again?

Query: Hello doctor, Eleven weeks ago, I had protected intercourse with two female prostitutes. I do not doubt the condom, as I remember taking it off (we used two different condoms for oral and vaginal sex). Still, in the second encounter, I clearly remember the partner placing the condom on me, but I rem...  Read Full »

Dr. Kiran Anaparthi

Answer: Hello, Welcome to Thank you for your query. The combination of HIV 4th generation test is a test to detect acute HIV infection, and reduced time from 45 to 90 days to as early as 15 to 45 days, because it uses p24 antigen and it appears in the blood after ten days of exposure if you ...  Read Full »

Could you please look into the reports and confirm whether the test carried out for HIV is a fourth-generation test?

Query: Hello doctor, Please see attached the reports for the viral markers (3H) and VDRL and RPR screening along with this query. These tests were undertaken after eight months and 19 days of exposure or 263 days post-exposure. I wanted to confirm if the test used in the attached report for HIV screening i...  Read Full »

Dr. Ravinder K. Manchanda

Answer: Hello, Welcome to The HIV (human immunodeficiency virus) test, which is performed, is a fourth-generation test. The fourth-generation test is a combo or a duo test. It looks for both antigens and antibodies of HIV. It is great that your test for HIV, HBV (hepatitis B virus), HCV (hepati...  Read Full »

Can protective non-penetrative sex cause tiredness, fever, and pain in my lymph nodes?

Query: Hello doctor, Two months back, I went to a sex worker had a protected blowjob and sucked her boobs. No anal or penetrative sex was performed. I did not feel sick after that for some time. A few weeks back, I went to a massage parlor where I had a body-to-body massage. She was lying on top of me. At ...  Read Full »

Dr. Rakesh Kumar Bahunuthula

Answer: Hello, Welcome to I understand your anxiety; it is natural to feel that way. But you need to remain calm and think in the right way. The risk of HIV (human immunodeficiency virus) transmission through blow job is negligible, and WHO (World Health Organisation) does not recognize this ...  Read Full »

I took the HIV combo test on the 32nd day after exposure. Is it a fourth-generation test?

Query: Hi doctor, I took the HIV-1, HIV-2, and p24 tests on the 32nd day after exposure. I am attaching the file. I want to know whether this is a fourth generation test. If yes, what are the chances that a fourth-generation test is negative on the 32nd day and will turn positive after the 45th day?  Read Full »

Dr. Ravinder K. Manchanda

Answer: Hi, Welcome to The answers to your queries are: The test which has been performed is a fourth-generation test. This test looks for HIV (human immunodeficiency virus) antigens and antibodies. The test result (attachment removed to protect the patient's identity) is negative. If this...  Read Full »

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