Tridot Test

Tridot test is used to detect the level of antibodies to HIV 1 and HIV 2 in the blood. It is used to confirm HIV infection after potential exposure. In addition, this test is also used to assess the progress of HIV treatment.

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I got tested for HIV after seven months of exposure from a diagnostic center. Can these results be trusted?

Query: Hello doctor, After exposure, I got tested for HIV (the condom covered half of the penis after ejaculating inside). All tests were conducted after seven months of exposure. Most of them are rapid tests, and two are CMIA tests from a diagnostic center. My query is, are these test results reliable? ...  Read Full »

Dr. Goswami Parth Rajendragiri

Answer: Hello, Welcome to the Following is my opinion for your asked question. I have seen the attached reports (attachments removed to protect the patient's identity). All your HIV (human immunodeficiency virus) test reports with different methods are negative for HIV. Diagnostic centers ar...  Read Full »

How accurate is the TRI-DOT test in detecting HIV positive?

Query: Hello doctor, I had intercourse with a sex worker seven months ago. I had double protection. But the condom broke in the middle, and I immediately took it out and did not expose it to her vaginal fluid. Within three hours, I took the TRI-DOT test, and it was negative. I have had body ache and fatig...  Read Full »

Dr. Thiyagarajan. T

Answer: Hi, Welcome to I can understand your concern. But I think that you are more anxious and depressed than you should be. You can repeat HIV (human immunodeficiency virus) test if that concerns you the most. For vitamin B12 deficiency, you can take a B12 tablet instead of an injection,...  Read Full »

Is it necessary to take fourth-generation test even if other HIV tests are negative?

Query: Hello doctor,I had protected exposure followed by P24 antigen after 33 days, fourth-generation P24 Ag, HIV 1 and 2 antibodies (CMIA) after 38 days, and then tridot after 93 days. All reports were negative for all the tests. As all the reports are negative and protected exposure, can my HIV status be...  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to The answers to your queries are: As you mentioned that it was protected sex, and there was no break or slippage of the condom. The risk of acquisition of HIV in this scenario is non-existent. It is great that your HIV test report at both time points is negative. If the...  Read Full »

Are tridot test results reliable after 5 months of exposure?

Query: Hello doctor, I had oral sex with an unknown woman for 4-5 seconds but then got scared and got away. I do not know her HIV status. I got a negative test on the seventh day and 158th day (5 months 10 days), which is also negative. I do not have any other exposure after that and in these five months....  Read Full »

Dr. Goswami Parth Rajendragiri

Answer: Hi, Welcome to You just had oral sex for 4 seconds and no intercourse at all. So you do not have any risk of HIV transmission as per history. However, it is good that you had done an investigation for HIV. Any HIV test after three months of exposure rules out HIV conclusively. You...  Read Full »

How accurate is the Tridot test result after 160 days of exposure?

Query: Hello doctor, I had oral sex with my partner for 4-5 seconds, then I got scared and got away. And no sexual intercourse. I did not have any symptoms like fever, sore throat, oral thrush, etc. I got tested after seven days and after 160 days Tridot test. Both came negative (Tridot was nonreactive on...  Read Full »

Dr. Basti Bharatesh Devendra

Answer: Hi, Welcome to The chances of transmission of HIV by the given description are almost nil unless there are bleeding wounds in the contact area. HIV antibodies test (here Tridot) is negative after three months of exposure is conclusive. You are free from HIV provided there is no furt...  Read Full »

Why is my HIV reference range close to borderline?

Query: Hello doctor,I want to know why my HIV test reference range is at a close borderline. i.e. 0.89. Is there any chance of getting HIV? I have tested this now, and my window period is over a year. Please clarify my doubt. I have tested HIV by rapid kits like tridot so many times after the window period...  Read Full »

Dr. Ashok Kumar Choudhary

Answer: Hello, Welcome to I read your query and understand your concerns. First of all, I like to assure you that test is non-reactive rather than borderline, which means that you have no active infection. Since almost one year crossed after the exposure (I assume your one year reference...  Read Full »

Are both CMIA and CLIA tests for HIV reliable?

Query: Hi doctor, I want to enquire about the HIV test and window period. My last exposure was six months back with CSW. It was protected, but I did HIV test on the 45th day, 75th day, 96th day, 120th day, and 168th day of last exposure due to fear. They did HIV tri-dot test, and all are negative. It is a...  Read Full »

Dr. Goswami Parth Rajendragiri

Answer: Hi, Welcome to the I can understand your concern. I will solve your doubt, so do not worry. Any test, including antibody testing, is useful to rule out HIV after six weeks and three months. CLIA (Clinical laboratory improvements amendments) and CMIA (Chemiluminescence micropartical im...  Read Full »

Are HIV rapid test results reliable after three months of exposure?

Query: Hello doctor, I did an HIV card test on 85th day after unprotected sex and it was negative. Again I went for HIV 1 and 2 rapid test on 97th day. It came non-reactive. Do I need any further test after six months? Or is it conclusive?  Read Full »

Dr. Basti Bharatesh Devendra

Answer: Hi, Welcome to The chances of transmission of HIV by unprotected sex with an HIV positive person is very low. The negative HIV antibodies test after three months of exposure is conclusive provided there is no further risk exposure. There is no need for further HIV tests.  Read Full »

Are results of tridot antibody conclusive?

Query: Hi doctor, I have an HIV test done at 45 days, 75 days, and 96 days. All are negative. Test I underwent is tridot antibody test. Is it conclusive? Or do I need to test more? CDC guidelines say conclusive at 90 days. So do I want to reconfirm? I tested at two famous labs which is NABL accredited....  Read Full »

Dr. Ravinder Kaur Sachdeva

Answer: Hi, Welcome to It is good that your 96 days post exposure test is negative. The answer to your queries are: If the last unprotected exposure was 96 days ago only and there had been no further exposure, your test would be considered conclusive in that scenario. But if there had bee...  Read Full »

HIV tridot antibody test negative till 10 weeks. Can it turn positive in 12 weeks?

Query: Hi doctor, HIV tridot antibody test done at 6 weeks, 8 weeks and 10.5 weeks. All negative. Are there chances it will turn positive at 12 weeks now? Test done with blood taken from vein.  Read Full »

Dr. Goswami Parth Rajendragiri

Answer: Hi, Welcome to Following is my opinion for your asked question. HIV tri dot antibody test is having good sensitivity and specificity in ruling out HIV as like that of fourth generation ELISA. Usually tri dot HIV test is able to rule out HIV after six weeks of exposure. However, HIV t...  Read Full »

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